Wu Zhongqi^1,2 , Zhou Yan^2,5 , Liu Panpan^1,2 , Wei Yanjun⁶ , Zhang Yan⁶ , Li Youzhi⁵ , Liu Shenkui¹ , Fang Xuanjun^1,2,3,4,5

1 Alkali Soil Natural Environmental Source Center, Northeast Forestry University, Harbin, 150040
2 Hainan Institute of Tropical Agricultural Resources, Sanya, 572025
3 Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji, 311800
4 Cuixi Academy of Biotechnology, Zhuji, 311800
5 College of Life Sciences and Technology, Guangxi University, Nanning, 530005
6 College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, 150040
Author    Correspondence author
Bt Research, 2016, Vol. 7, No. 1   doi: 10.5376/bt.2016.07.0001
Received: 16 Nov., 2016    Accepted: 20 Nov., 2016    Published: 22 Nov., 2016

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Wu et al., 2016, Draft genome sequence of Bacillus thuringiensis strain S3076-1, Bt Research, Vol.7, No.1 1-7 (doi: 10.5376/bt.2016.07.0001)

Abstract　Bacillus thuringiensis S3076-1 can produce some parasporal crystal with a variety of forms, which may have insecticidal activity to Lepidoptera insects. In this study, the de novo whole genome sequence of Bt S3076-1 was carried out by employing Illumina HiSeq 2000 and generated 1.42 Gb raw data. Through the original reads data quality control, filtering and SOAPdenovo short sequence assembly to get a total of 231 scaffolds, the genome size was 6.42 Mb, N50 length was 97325 bp, GC content of 34.81%. The genomic information of 14 Bt strains in NCBI database were selected as the reference, and the genomic sequences of Scaffolds were assembled to obtain the 3 replicators. Among them, the size of the genome was 5.72 Mb, the content of GC was about 35.00%, and the length of the genomic sequences of the four plasmids were 181 kb and 518 kb, and the GC contents were 33.10% and 33.13%, respectively. Based on the genome sequence and annotation information, the genome of the visual sketch was created by GCviewer server. The whole genome sequencing and construction of Bt S3076-1 provide the basis for the functional study of the strain, and contribute to the identification of Bt S3076-1 insecticidal toxin protein.

Bacillus thuringiensis; Bt S3076-1; Whole genome sequencing; Genome assembling; Draft genome